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Image Search Results
Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Article Title: Neutrophil extracellular trap fragments stimulate innate immune responses that prevent lung transplant tolerance
doi: 10.1111/ajt.15163
Figure Lengend Snippet: (a) Sham operated B6 mice or ischemically-injured Balb/c → B6 lung recipients following indicated treatment (N=5 / group) were assesed for lung function at 90 minutes after reperfusion. Ischemically-injured Balb/c → B6 lung recipients following indicated treatments were assed for NETs within circulating plasma as measured by (b) ELISA-based detection of MPO associated DNA complexes and (c) total double stranded DNA quantitation following treatment with saline, DNAse, Cl-Amidine or Cl-Amidine & DNAse. (d) Balb/c→ B6 lung recipients (N=5 / group) BALF 1.5 hours after reperfusion were quantified by ELISA for citrullinated histone (Cit-H3b) associated DNA complexes 1.5 hours after engraftment. (e) B6 mice following clamping of the right hilum to induce left lung warm ischemic injury received either saline or indicated doses of DNAse 30 minutes after reperfusion. 1.5 hours after hilar clamping BAL fluid was collected and analyzed for cit-H3b DNA complexes. Where indicated means with ± standard deviation (S.D.) are shown with n.s as non-significant, **p< 0.01 and ****p <0.0001.
Article Snippet: NETs release in culture and plasma was quantified with Quanti-IT Pico Green double stranded (
Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Saline, Standard Deviation